FectoVIR®-AAV
Available at research and GMP grade to intensify production of recombinant AAV rAAV viral vectors at any scale from benchtop to 2000L scale bioreactor...
Overview
pPLUS® AAV-Helper is a novel helper plasmid designed for AAV production. This plasmid is the outcome of an extensive R&D program based on our proprietary DNA assembly technology (e-Zyvec® technology) aiming at exploring the synergies of multiple genetic features to improve the infectivity (measured in TU/mL) and quality (full/empty ratio) of viral particles obtained through triple transfection of suspension HEK-293 cells with FectoVIR®-AAV.
pPLUS®-AAV Helper is readily available as an off-the-shelf product in both R&D Grade and HQ Grade.
GMP Grade can be ordered through custom production – please inquire
An innovative construct
pPLUS® AAV-Helper is a novel Helper plasmid integrating new helper elements in the sequence leading to improved AAV particles infectivity. The plasmid size (13.3 kb) has been reduced compared to a standard Helper plasmid and the sequence is free of Hexon Associated Precursor and Fiber sequences.
Strong improvement of AAV productivity and infectivity when used together with FectoVIR®-AAV
The use of pPLUS®-AAV Helper leads to improved titers (VG/mL) and infectivity (TU/mL) compared to other commercial pHelper, irrespective of transfection reagent used. A synergistic effect is obtained when pPLUS® AAV-Helper and FectoVIR®-AAV are used together with up to 4-fold improvement in VG titers and 8-fold improvement in TU titers.
Efficiency with all tested AAV serotypes (AAV2, AAV5, AAV8, AAV9)
The positive impact on titers and infectivity has been observed with various serotypes confirming the suitability of the pPLUS® AAV-Helper for production of AAVs commonly used in the clinic.
Consistent performance across cell lines
pPLUS® AAV-Helper has shown consistent performance when used with various cell lines commonly found in AAV production processes confirming its suitability for use in various production systems.
High Quality (HQ) Grade available
pPLUS® AAV-Helper is available off-the-shelf in HQ Grade to further support Gene Therapy manufacturers in their projects.
PEIpro®
PEIpro® is the leading PEI-based DNA transfection reagent that offers flexibility and scalability for viral vector manufacturing.
Plasmid Engineering Service
Polyplus provides an integrated service for design and production of tailor-made DNA plasmid through our unique and Innovative assembly technology.
Plasmid Manufacturing Service
Polyplus offers a comprehensive range of plasmid manufacturing services from DNA plasmid design to plasmid DNA manufacturing and analytical testing.
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