Available at research and GMP grade to intensify production of recombinant AAV rAAV viral vectors at any scale from benchtop to 2000L scale bioreactor...
pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid is the outcome of an extensive R&D program based on our proprietary DNA assembly technology (e-Zyvec® technology) aiming at exploring the synergies of multiple genetic features to improve the infectivity (measured in TU/mL) and quality (full/empty ratio) of viral particles obtained through triple transfection of suspension HEK-293 cells with FectoVIR®-AAV.
pPLUS®-AAV Helper is readily available as an off-the-shelf product.
An innovative construct
pPLUS® AAV-Helper is a novel Helper plasmid integrating new helper elements in the sequence leading to improved AAV particles infectivity. The plasmid size (13.3 kb) has been reduced compared to a standard Helper plasmid and the sequence is free of Hexon Associated Precursor and Fiber sequences.
Strong improvement of AAV productivity and infectivity when used together with FectoVIR®-AAV
The use of pPLUS®-AAV Helper leads to improved titers (VG/mL) and infectivity (TU/mL) compared to other commercial pHelper, irrespective of transfection reagent used. A synergistic effect is obtained when pPLUS® AAV-Helper and FectoVIR®-AAV are used together with up to 4-fold improvement in VG titers and 8-fold improvement in TU titers.
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