pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid is the outcome of an extensive R&D program based on our proprietary DNA assembly technology (e-Zyvec® technology) aiming at exploring the synergies of multiple genetic features to improve the infectivity (measured in TU/mL) and quality (full/empty ratio) of viral particles obtained through triple transfection of suspension HEK-293 cells with FectoVIR®-AAV. 

pPLUS®-AAV Helper is readily available as an off-the-shelf product. 

Ordering Information

Reference NumberQuality Grade Packsize
101000181Research grade1mg
101000182Research grade10mg
101000183Research grade100mg

Minimum Ordering Quantity: 5mg 

Want to request a quote

Get Now


An innovative construct

pPLUS® AAV-Helper is a novel Helper plasmid integrating new helper elements in the sequence leading to improved AAV particles infectivity. The plasmid size (13.3 kb) has been reduced compared to a standard Helper plasmid and the sequence is free of Hexon Associated Precursor and Fiber sequences.

Strong improvement of AAV productivity and infectivity when used together with FectoVIR®-AAV

The use of pPLUS®-AAV Helper leads to improved titers (VG/mL) and infectivity (TU/mL) compared to other commercial pHelper, irrespective of transfection reagent used. A synergistic effect is obtained when pPLUS® AAV-Helper and FectoVIR®-AAV are used together with up to 4-fold improvement in VG titers and 8-fold improvement in TU titers.

Efficiency with all tested AAV serotypes (AAV2, AAV5, AAV8, AAV9)

The positive impact on titers and infectivity has been observed with various serotypes confirming the suitability of the pPLUS® AAV-Helper for production of AAVs commonly used in the clinic.

Consistent performance across cell lines

pPLUS® AAV-Helper has shown consistent performance when used with various cell lines commonly found in AAV production processes confirming its suitability for use in various production systems.

Other sources to help you understand pPLUS® AAV-Helper better

Other Sources

Check our FAQs

These FAQs are organized by application to guide you to find the best answer possible.

Product Documents

You have access to all the documents related to the transfection reagent.

Polyplus Database

Search for publications in our Transfection Database with Polyplus transfection reagents


This lexicon will help you to understand the different terms related to Polyplus-transfection®.

Learn more



Easy – rAAV pTransgene Plasmid
3-step plasmid engineering service for complex plasmids
e-Zyvec® Technology


Webinar replay: Next Generation of Genetic Engineered AAV Helper Plasmids


pPLUS® AAV-Helper in a nutshell
Poster : Novel engineered pHelper plasmid to improve yield and quality of several AAV serotypes in suspension cell culture systems

View All Resources