FectoCHO® Expression System

Specifications
Overview
Capabilities
All Data
Resources

Specifications

Product	FectoCHO^® Expression system
Application	Protein / Antibody Production
Kit components	FectoCHO^® CD Expression Medium FectoPRO^® Transfection reagent FectoPRO^® Expression Booster
Cell types	Suspension CHO cells such as CHO-K1, CHO-S or ExpiCHO™-S
Number of transfections	1 mL of FectoPRO^® Transfection reagent is sufficient to transfect 800 ml to more than 1 liter of FectoCHO^® CD Expression Medium
Storage	Store FectoPRO^® and FectoCHO^® CD Expression Medium at 5 °C ± 3°C. Do not freeze. Expiry date is indicated in the certificate of analysis (available in "My account") and on the product.

Overview

The FectoCHO® Expression system, consists in the synergistic association of a novel CHO chemically defined medium, FectoCHO® CD Expression medium and a powerful transfection reagent, FectoPRO®.

FectoCHO® CD Expression medium is optimised to facilitate adaptation and cultivation of various strains of CHO cells such as CHO-K1, CHO-S and ExpiCHO™-S : extensive sequential adaptation is not required. FectoPRO®-mediated transient transfection in this chemically defined medium is optimal and therefore leads to the highest protein and antibody yields using Transient Gene Expression; with up to 4 times higher protein yields than with the competitors’ systems.

Ordering Information

Reference Number	Expression Medium	Transfection Reagent	Expression Booster
101000011	FectoCHO® CD Medium 1 L	FectoPRO® 1 mL	1mL
101000003	FectoCHO® CD Medium 6 x 1 L	-	-
101000014	-	FectoPRO® 1 mL	1mL
101000007	-	FectoPRO® 10 mL	10mL
101000019	-	FectoPRO® 100 mL	4x10mL
716-01LKIT	1 L	1 mL	1 mL
716-06L	6 x 1 L	-	-
116-001	-	10 mL	10 mL
116-010	-	10 mL	10 mL
116-040	-	4 x 10 mL	4 x 10 mL
301000270	*NO DISPLAY* FectoCHO

1 mL of FectoPRO® Transfection Reagent is sufficient to transfect 800 ml to more than 1 liter of FectoCHO® CD Expression Medium. Bulk quantities are available upon request.

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Capabilities

Outstanding protein production yields in suspension CHO cells reaching up to 4 times compared to competitors’ systems.

The combination of a chemically defined medium optimized for transient transfection and protein expression, FectoCHO® CD Expression medium, and of a highly efficient transfection reagent, FectoPRO® leads to outstanding protein production yields in various CHO cells, such as CHO-S and CHO-K1 cells.

Ideal for high density cell systems such as ExpiCHO™-S cells

FectoCHO® Expression system is particularly well suited for high density cells, such as the ExpiCHO™-S cells. FectoCHO® CD medium is able to sustain growth and viability at very high cell density for up to 10 days, while ensuring a higher transfection efficiency with FectoPRO®, leading to very high protein yields in ExpiCHO™-S cells.

Excellent CHO cell adaptation

FectoCHO® CD Expression medium allows for CHO cells to reach high cell density, while maintaining an excellent viability with short doubling time. This medium has been specifically developed to allow a quick cell adaptation, whatever the CHO cell type used.

Chemically defined kit components

FectoCHO® expression system is a 3 component kit optimized to work together: FectoCHO® CD Expression Medium, FectoPRO® Transfection Reagent and FectoPRO® Expression Booster. All of the above being chemically defined components for a convenient use in biomanufacturing processes.