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FectoCHO® Expression system
Protein / Antibody Production
FectoCHO® CD Expression Medium
Suspension CHO cells such as CHO-K1, CHO-S or ExpiCHO™-S
|Number of transfections|
1 mL of FectoPRO® Transfection reagent is sufficient to transfect 800 ml to more than 1 liter of FectoCHO® CD Expression Medium
Store FectoPRO® and FectoCHO® CD Expression Medium at 5 °C ± 3°C. Do not freeze.
The FectoCHO® Expression system, consists in the synergistic association of a novel CHO chemically defined medium, FectoCHO® CD Expression medium and a powerful transfection reagent, FectoPRO®.
FectoCHO® CD Expression medium is optimised to facilitate adaptation and cultivation of various strains of CHO cells such as CHO-K1, CHO-S and ExpiCHO™-S : extensive sequential adaptation is not required. FectoPRO®-mediated transient transfection in this chemically defined medium is optimal and therefore leads to the highest protein and antibody yields using Transient Gene Expression; with up to 4 times higher protein yields than with the competitors’ systems.
|Reference Number||Expression Medium||Transfection Reagent||Expression Booster|
|101000011||FectoCHO® CD Medium 1 L||FectoPRO® 1 mL||1mL|
|101000003||FectoCHO® CD Medium 6 x 1 L||-||-|
|101000014||-||FectoPRO® 1 mL||1mL|
|101000007||-||FectoPRO® 10 mL||10mL|
|101000019||-||FectoPRO® 100 mL||4x10mL|
|716-01LKIT||1 L||1 mL||1 mL|
|716-06L||6 x 1 L||-||-|
|116-001||-||10 mL||10 mL|
|116-010||-||10 mL||10 mL|
|116-040||-||4 x 10 mL||4 x 10 mL|
|301000270||***NO DISPLAY*** FectoCHO|
Outstanding protein production yields in suspension CHO cells reaching up to 4 times compared to competitors’ systems.
The combination of a chemically defined medium optimized for transient transfection and protein expression, FectoCHO® CD Expression medium, and of a highly efficient transfection reagent, FectoPRO® leads to outstanding protein production yields in various CHO cells, such as CHO-S and CHO-K1 cells.
Ideal for high density cell systems such as ExpiCHO™-S cells
FectoCHO® Expression system is particularly well suited for high density cells, such as the ExpiCHO™-S cells. FectoCHO® CD medium is able to sustain growth and viability at very high cell density for up to 10 days, while ensuring a higher transfection efficiency with FectoPRO®, leading to very high protein yields in ExpiCHO™-S cells.
Chemically defined kit components
FectoCHO® expression system is a 3 component kit optimized to work together: FectoCHO® CD Expression Medium, FectoPRO® Transfection Reagent and FectoPRO® Expression Booster. All of the above being chemically defined components for a convenient use in biomanufacturing processes.
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