pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in s...
Hear from Eric Mauro, plasmid engineering scientist, Polyplus, and Goutham Kumar Ganjam, head of manufacturing, DiNAMIQS, as they discuss Polyplus’ state-of-the art approach to design new helper plasmids (phelpers) with the aim of improving both the infectiosity (TU/mL) and the quality (full/empty ratio) of the viral particle in suspension systems. Our experts will also highlight how DiNAMIQS evaluated these novel helper plasmids in their manufacturing platform and what was learned about the potential to improve both productivity and quality of rAAV produced.
Key Learning Objectives:
- Strategies to improve AAV helper plasmid engineering.
- Role of engineering of helper plasmid to improve AAV yield and quality.