pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in s...
siRNA, miRNA (microRNA), and other oligonucleotides: pre-miRNA, mimic miRNA, antimiR…
Transfection of siRNA and other oligonucleotides
Adherent and suspension cells
|Number of transfections|
1 ml of INTERFERin® is sufficient to perform 500-1000 transfections in 24-well plates.
Store INTERFERin® at 5 °C ± 3°C.
INTERFERin® provides very high silencing efficiency already at 1 nM siRNA and can be used in a wide variety of adherent and suspension cells. Using low concentration of siRNA avoids off-target effects, and its gentle mode of action ensure more robust data and excellent cell viability. Easy to use thanks to its compatibility with serum and antibiotics, INTERFERin® is also perfectly suited for transfection of miRNA and other oligonucleotides like pre-miRNA, mimic miRNA, antimiR.
Less siRNA, less off-target effects
Publications have shown that transfecting high siRNA concentrations can lead to non-specific off target effects. The reliability of the experimental data can be increased by using as little siRNA as possible. This is why INTERFERin® has been specifically designed to provide high silencing efficiency using low siRNA concentrations. INTERFERin®-mediated delivery of 1 nM of a specific siRNA shows selective and highly efficient knockdown of gene expression, while a competitor (L2K) needs at least 10 nM siRNA to reach 50% silencing efficiency.
Suitable for miRNA transfection
Transfection of microRNA (miRNA) oligonucleotides is increasingly being used to analyze biological effects of specific miRNAs on cell function. INTERFERin® is the reagent of choice for delivering miRNA, miRNA mimics or pre-miRNAs. INTERFERin® is the latest generation siRNA & miRNA transfection reagent, especially designed for high transfection efficiency in a wide variety of cells, resulting in high gene silencing or stimulation of gene expression. Indeed, some miRNA are also known to induce gene expression by association with the promoter of the gene of interest. For example, INTERFERin®-mediated delivery of miR-373 leads to a 3 fold increase in E-Cadherin expression.
Take control of gene silencing
The delivery of RNAi machinery is a powerful tool to explore treatment options for many diseases and also in furthering our understanding of mechanistic interplay in cellular signalling pathways and functionality. INTERFERin® has a proven track record of highly effective RNAi delivery in primary cells and cell lines, and is thus a competitive and reliable choice for future genetic and biological research.
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