in vivo-jetPEI®
in vivo-jetPEI® is a ready-to-use cationic polymer reagent recommended for in vivo transfection of DNA, siRNA, miRNA, shRNA and other oligonucleotide...
Specifications
| Type | Ready-to-use lipid-based nanoparticles |
|---|---|
| Molecule delivered | mRNA, saRNA, gRNA |
| Biodistribution & Injection routes | Universal biodistribution: Any organ or tissue (depending on the injection route) |
| Number of injections in mice | 1 mL is sufficient to perform 50 intravenous injections or 100 intramuscular injections in mice |
| Storage | Store in vivo-jetRNA®+ at 5 ± 3°C. Do not freeze. |
| Provided with | mRNA buffer |
Overview
in vivo transfection reagents are the most powerful alternative to viral vectors for nucleic acid delivery. They are easy to use, cost-effective and considered as safe and efficient vehicles for RNA delivery. mRNA transfection is rapidly emerging as a promising method for nucleic acid-based therapy and offers an attractive substitute to plasmid DNA. Non-viral mRNA delivery methods have already proven their efficiency in vaccination through antigen presenting cells modification and in anti-cancer therapy by directly targeting malignant cells. The intrinsic advantage of mRNA-based immunotherapy relies on the self-adjuvant activity of mRNA and the fact that small amounts of encoded antigen are sufficient to obtain robust immune response.
in vivo-jetRNA®+ is a non-viral delivery solution composed of pre-formed lipid-based nanoparticles. It was specifically developed for in vivo mRNA delivery. Its high delivery efficiency combined with its safety make in vivo-jetRNA®+ an innovative alternative to lipid nanoparticles (LNPs), with the added advantage of being user-friendly with a 2-step protocol.
in vivo-jetRNA®+ can be used to deliver mRNA, saRNA and gRNA via systemic or local administration routes in various animal models, making it an ideal tool for scientists developing vaccines, protein replacement therapies, performing oncology studies, or working on genome editing. It has also proved highly effective for ex vivo applications, such as the generation of mRNA CAR-T cells.
Next generation lipid-based delivery for mRNA therapeutics: in vivo-jetRNA®+
Efficient: 100% mRNA encapsulation leading to delivery results comparable to LNPs
in vivo-jetRNA®+ is the reagent of choice for delivering mRNA in vivo. This is due to its intrinsic properties: it protects its payload from ubiquitous endonucleases, prevents non-specific interactions with proteins and promotes efficient cell entry. in vivo-jetRNA®+ shows an astonishing ability to encapsulate 100% of the mRNA, leading to efficient gene expression comparable to that of lipid nanoparticles (LNPs) currently used as the gold standard for non-viral delivery.
Time-saving: Ready-to-use reagent requiring no equipment or formulation expertise
LNPs are widely used for mRNA delivery, but their optimal formulation requires expertise, months of work and specific equipment and consumables. With in vivo-jetRNA®+, there is no need to spend time and budget on formulation, as it is a ready-to-use optimised formulation with a simple protocol. Indeed, the mRNA/in vivo-jetRNA®+ lipid-based nanoparticles solution is prepared in only two steps and is ready to be injected into the animal within 15 minutes. Besides, it does not require any formulation equipment. Our friendly Scientific Support team is happy to provide protocols tailored to your needs.
Stable: Lipid-based nanoparticles are very stable over time
In addition to its ability to encapsulate mRNA, in vivo-jetRNA®+ excels in stability when formulated with mRNA. Indeed, mRNA/in vivo-jetRNA®+ lipid-based nanoparticles retain their transfection efficiency for 4 weeks when stored at 4°C and for 72 hours when stored at RT. Additionally, while mRNA concentration influences nanoparticle size, in vivo-jetRNA®+ maintains a stable size over time, whether the mRNA concentration is low or high.
Universal: Target any organ/tissue depending on the administration route
The choice of route of administration results in a different biodistribution of the mRNA. For example, administration of mRNA/in vivo-jetRNA®+ nanoparticles by intraperitoneal injection results in gene expression in many organs, including the spleen and lymph nodes which play a major role in the immune response, whereas intramuscular injection induces a more localized mRNA expression. in vivo-jetRNA®+ can be administered systemically or locally, allowing you to modulate biodistribution according to your needs and objectives.
Safe: Keep animals and organs healthy and does not trigger pro-inflammatory response
in vivo-jetRNA®+ does not induce side effects after injection, with all animals remaining healthy and no pain being observed. Also, the organ(s) of interest remain(s) phenotypically intactintact, and no tissue damage is observed, regardless of the route of administration. Furthermore the use of in vivo-jetRNA®+ does not trigger the expression of pro-inflammatory cytokines. As a non-viral delivery reagent, in vivo-jetRNA®+ has several advantages over conventional vaccine approaches: it is safer for recipients, faster and cheaper to produce, and has great potential to address many unmet medical needs.
jetMESSENGER®
jetMESSENGER® is a designed for high mRNA transfection efficiency in primary cells, cancer cell lines, neurons, and stem cells.
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