pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in s...
jetOPTIMUS® DNA Transfection Reagent
Transient and stable gene expression from plasmid DNA transfection
Adherent cells and hard-to-transfect cells:
|Number of transfections|
1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol
Store jetOPTIMUS® at 5 °C ± 3°C.
jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells resulting in higher transfection efficiency, even in hard to transfect cells. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression with minimal impact on cell viability and morphology.
jetOPTIMUS® is highly efficient for DNA delivery for various applications, such as transient and stable gene expression, CRISPR genome editing using DNA approach, as well as virus production in small scale.
|Reference number||Reagent size||Buffer size|
|101000051||jetOPTIMUS® 0.1 mL||10 mL|
|101000025||jetOPTIMUS® 0.75 mL||2 x 60 mL|
|101000006||jetOPTIMUS® 1.5 mL||4 x 60 mL|
|201000001||-||jetOPTIMUS® Buffer 60 mL|
|201000032||-||jetOPTIMUS® Buffer 10 mL|
|117-01||0.1 mL||10 mL|
|117-07||0.75 mL||2 x 60 mL|
|117-15||1.5 mL||4 x 60 mL|
Superior gene expression in easy-to-hard-to-transfect cells
jetOPTIMUS® improves DNA transfection efficiency in easy- and difficult-to-transfect cells used as in vitro cell culture models. Tested on various primary cells and cancer cell lines, jetOPTIMUS® proved its superiority by reaching higher transfection efficiencies and gene expression than main competitors.
Excellent cell viability and morphology
Reaching high transfection efficiencies is often at the expense of cellular integrity, with adverse effects on cell cycle, metabolism and signaling pathways. At Polyplus-transfection, we make no compromise on cell integrity: jetOPTIMUS® transfection reagent is gentle on cells, preserving cell viability and morphology, which allows generation of biologically relevant data from gene expression studies.
Time-saving with optimized ready-to-use protocol
jetOPTIMUS® is a ready-to-use transfection reagent provided with its own complexation buffer (jetOPTIMUS® Buffer). The protocol is optimized for simplicity of use (all plate sizes), culture medium compatibility (antibiotics, serum) and cost-effectiveness (lowest amount of DNA and volume of reagent).
Obtain accurate and reliable full length plasmid sequencing with our Next Generation Sequencing service.
Polyplus provides an integrated service for design and production of tailor-made DNA plasmid through our unique and Innovative assembly technology.
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