Ready-to-use cationic polymer

Molecules delivered

DNA, siRNA, miRNA, shRNA, mRNA, saRNA or other oligonucleotides

Biodistribution and Injection routes

Universal biodistribution: Any organ or tissue (depending on the injection route)
Various administration routes: Both systemic or local routes can be used

Number of injections

100 µl of in vivo-jetPEI® is sufficient to perform 15 to 25 intravenous injections in mice


Store in vivo-jetPEI® at -20 ± 5°C.
Expiry date is indicated in the Certificate of Analysis and on the product.

Product documentation

Certificate of Analysis (CoA)
Material Safety Data Sheet (MSDS)

Regulatory documentation

With GMP grade only:
Certificate of Compliance (CoC)
Drug Master File (DMF) submitted to FDA
Chemistry, manufacturing and controls (CMC) documentation
Quality agreement


in vivo-jetPEI® is a ready-to-use cationic polymer reagent used for in vivo transfection of nucleic acids (DNA, siRNA, miRNA, shRNA and other oligonucleotides) in any animal model.

With more than 600 peer-reviewed publications, in vivo-jetPEI® is established in the field of in vivo delivery, whether for functional studies, oncology research as well as vaccination purposes. Additionally, in vivo-jetPEI® has the advantage of being available in 2 different quality grades (Research and GMP grades) to support our customers at each step of their development process.

Ordering Information

Reference NumberAmount of reagentAmount of 10% glucose solution
101000040in vivo-jetPEI® 0.1 mL10ml
101000030in vivo-jetPEI® 0.5 mL2 x 10ml
102000003in vivo-jetPEI® GMP powder 10 gnone

Bulk quantities and GMP grade reagent are available upon request. Please contact us.


Want to request a quote

Get Now


Polyvalent: in vivo delivery of DNA or si/miRNA through any commonly used administration routes

in vivo-jetPEI® is effective for the delivery of nucleic acids (DNA, siRNA/miRNA, shRNA, other oligonucleotides) in any animal model (mouse, rat, rabbit, llama, monkey, etc.) and can be used to target a wide range of organs by systemic and local administration. The route of injection largely determines which organs are targeted, allowing scientists to modulate biodistribution according to their needs and objectives. When delivering pDNA, cell-specific promoters can also be combined with a local injection route to restrict expression to a specific organ/tissue.

Time-saving: Ready-to-use reagent requiring no equipment or formulation expertise 

in vivo-jetPEI® is the reagent of choice to deliver various types of nucleic acid through most systemic and local injection routes.

Besides, with in vivo-jetPEI®, there is no need to spend time and budget on formulation, as it is a ready-to-use reagent with a straightforward protocol. Indeed, the nucleic acid and in vivo-jetPEI® complexes are prepared in only two steps and are ready to be injected into the animal model within 15 minutes.

Our friendly Scientific Support team is happy to provide protocols tailored to your needs.

Contact Scientific Support


GMP-compliant grade: in vivo-jetPEI® enables a seamless transition from fundamental research to Human clinical trials

In addition to being used for in vivo functional and proof-of-concept studies, in vivojetPEI® has been selected as a delivery vehicle for several drug development programs due to its reliability, safety and efficacy. Several preclinical studies and clinical trials are currently underway for cancer therapy, vaccination and immunization using the GMPcompliant quality of in vivojetPEI®. 

Safe: non-viral delivery method that does not trigger major inflammatory reaction nor hepatotoxicity

in vivojetPEI® constitutes a safe excipient for nucleic acid delivery. Following administration of in vivojetPEI®/nucleic acid complexes, there is no induction of major pro-inflammatory cytokines and no increase in sera levels of hepatic enzymes. (Bonnet et al., (2008), Pharm Res 25:2978-82). 

Renowned: Advanced in vivo delivery technology for functional studies, cancer research, immunization and vaccination

in vivo-jetPEI® is a powerful polymer-based reagent with unique properties. In the provided complexation solution, in vivo-jetPEI® condenses any nucleic acid into stable nanoparticles which are sufficiently small to efficiently diffuse within tissues and enter cells by endocytosis, while protecting naked nucleic acids from degradation. At the cellular level, in vivo-jetPEI® facilitates both endosomal escape via proton sponge mechanism (Akinc et al. (2005), J Gene Med 7: 657), and crossing of the nuclear membrane (Brunner et al. (2002), Mol Ther 5: 80). 

These properties make it the non-viral vector of choice for in vivo nucleic acid delivery, as nicely illustrated by its use in over 600 peer-reviewed publications.  

Accelerate Your In Vivo Research Studies

Did you obtain promising results in vitro and you now would like to go further and perform in vivo studies? 

in vivo-jetPEI® can meet your needs! Thanks to its versatility and ease of use, in vivo-jetPEI® is considered the gold standard in off-the-shelf solutions for in vivo delivery of nucleic acid delivery. Furthermore, in vivo-jetPEI® is available in both Research-grade and in vivo-jetPEI® GMP, which is manufactured in compliance with GMP standards to support your development from Discovery to Patients!

All Data

Looking for a Product Protocol or Certificate of Analysis

Create an Account

Other sources to help you understand in vivo-jetPEI® better

Other Sources

Check our FAQs

These FAQs are organized by application to guide you to find the best answer possible.

Product Documents

You have access to all the documents related to the transfection reagent.

Polyplus Database

Search for publications in our Transfection Database with Polyplus transfection reagents


This lexicon will help you to understand the different terms related to Polyplus-transfection®.

Learn more



Best Practices for Selecting Controls in Gene Expression Experiments
Origin Of Replication In Plasmids
Promoter Analysis | How to Decide Which Promoter To Use?


in vivo delivery from study to gene therapy
CRISPR-Cas9: overview, challenges and latest technologies


Poster : in vivo-jetPEI®, an alternative to lipid-based reagents or viral vectors for nucleic acid-mediated therapies
Poster : Non-viral transfection in mice: Guidelines for Nucleic Acid Delivery Experiments in Mice

View All Resources