pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in s...
|Number of transfections|
1.5 ml of jetMESSENGER® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates
Store jetMESSENGER® at 5 °C ± 3°C.
jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. jetMESSENGER® has been specifically designed to offer outstanding transfection efficiency in cells that are usually difficult to transfect, such as primary cells, cancer cell lines, neurons, and stem cells. jetMESSENGER® can also be used on a wide variety of easy to transfect cells. Transfection with jetMESSENGER® leads to very low cytotoxicity as it requires low amounts of mRNA and low volumes of reagent.
jetMESSENGER® is perfectly suited for gene expression, CRISPR gene editing, iPS generation, stem cell differentiation and immunotherapy assays.
Outperforms DNA transfection
jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:
- no risk of genome integration, hence no genome modification of the transfected cell
- no promoter regulation issue
- no need to reach the nucleus for efficient expression
- more gentle process
Allows higher gene expression than main competitors on a variety of cell lines
jetMESSENGER® is extremely efficient especially in hard-to-transfect cells compared to other mRNA transfection reagents. Transfection with jetMESSENGER® leads to outstanding mRNA delivery in a wide variety of cell lines, such as neurons, primary cells, stem cells and various cancer cell lines,
Extremely gentle on cells
By eliminating the need to reach the nucleus for efficient expression, jetMESSENGER® allows transfection of quiescent and slow dividing cells. In addition, jetMESSENGER® operates through an extremely gentle process. Cell viability remains extremely high during transfection and cell morphology is maintained.
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