We deliver “discovery grade*” plasmids as circular, double stranded DNA molecules, in a screw-cap plastic tube, re-suspended in 1X AE buffer (miniprep.) or 1X TE buffer (midi, maxi, giga prep.), at room temperature. Please note that we DO NOT provide bacterial stock.

Discovery grade plasmids can be ordered at multiple formats, please inquire for more information.

For larger scale or higher quality grade plasmid production please inquire Plasmid Manufacturing Service

*Discovery grade means that while plasmids are produced in a sterile environment according to good laboratory practice, quality control are limited to basic quality and identity test. Discovery grade plasmids should be restricted to Research Application.

For most projects, you do not need to send us existing plasmids or other DNA material. Desired sequences can be generated using DNA synthesis (outsourced).

However, some project may require you to send us DNA material (plasmid templates or purified gDNA, only) that we can use to overcome technical complexity, to ensure technical continuity or simply to reduce cost.

In this case, please follow these instructions:

• Use a 1.5 ml plastic tube, hermetically closed (screw cap or parafilm)
• Label the tube clearly
• Minimal volume: 15µL
• Minimal concentration: 100ng/µL
• Room temperature, please avoid dry ice.

Tubes should be sent at:

Polyplus-Transfection
Production Team
80 rue du Dr Yersin
59120 Loos
FRANCE

Please note that before starting any project, every plasmid template will be fully sequenced by NGS.

For gDNA samples please send a picture of an agarose gel to evaluate the integrity of the sample.

We have been successful in assembling plasmid up to 30Kb. Larger size plasmid can be considered as difficult to amplify in regular competent E. coli strains. However, feel free to reach out if your project requires such large plasmid as specific solutions may applied.

Upon receipt, briefly spin down the tubes containing your engineered plasmids (bench centrifuge) and store them at:

•  4°C for short storage (<1 month)
• – 20°C for long storage

We strongly recommend to aliquot the Polyplus discovery grade plasmids for long storage and to avoid freeze-thaw cycles.

Duration of storage are given here as indication. You should always control the integrity of long stored plasmids by running gel electrophoresis (denaturation) or sequencing (sequence integrity).

We do offer a pDNA amplification service for either engineered plasmids we generated, or your own plasmid you may wish to send us.

Amplification can be performed at small scale (up to several mg) and discovery grade, or at large scale (up to several gram) at R&D, High Quality or GMP grade with our Plasmid Manufacturing Service page.