pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in s...
Protein; Antibody; Peptide
Adherent & suspension cells grown in presence of serum
|Number of transfection|
0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
Store PULSin® at 5 °C ± 3°C. Do not freeze.
Hepes Buffer (20mM) for protein dilution; R-Phycoerythrin used as a positive control
The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.
PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.
PULSin® efficiently delivers antibodies in live cells
Antibodies are successfully delivered in mammalian cells and able to recognize their target protein inside the cytoplasm. PULSin® enabled the delivery of FITC-labeled anti-alpha-tubulin to the cytoplasm of high percentage of HeLa cells (85%). Similarly, anti-giantin Alexa Fluor® 488 was delivered to the cytoplasm of 98% of live HeLa cells, labeling the Golgi apparatus.
Simple protocol for successful protein delivery.
PULSin® will save you time and efforts compared to other techniques using viral transduction or chemical conjugation. PULSin® reagent is ready-to-use and provided with a dilution buffer and a fluorescent control protein (R-phycoerythrin).
The protocol is fast: simply add the reagent to the protein, incubate and add to the cells. Cells can be analyzed starting 4 hours post-delivery.
Easy to use alternative to chemical conjugation and viral transduction.
Comparison of PULSin® with two other protein delivery reagents shows a higher efficiency of protein delivery, Moreover, the amount of protein delivered per cell is higher with PULSin® as measured for R-phycoerythrin protein and for FITC-alpha-tubulin antibody (cf additional data).
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