jetMESSENGER® is a designed for high mRNA transfection efficiency in primary cells, cancer cell lines, neurons, and stem cells.
Messenger -transfection®, Vectura, 75 rue Marguerite Perey, 67400 Illkirch, France RNA transfection is a new solution for a wide variety of cells that are difficult to transfect with plasmid DNA including adherent (neurons or primary cells) and suspension cells (lymphocytes). This process has many advantages over DNA transfection: high percentage of transfected cells,
faster protein expression following transfection, and no risk of insertional mutagenesis in contrast to plasmid or viral vectors. In fact, mRNA is delivered and expressed in the cytoplasm and does not require to cross the nuclear membrane, one of the limiting steps in plasmid transfer. Furthermore, the last generation of modified mRNA reduces the toxicity and immune response usually induced by mRNA entry into the cytosol (1, 2). The transient nature of mRNA transfection is desirable for a number of applications, including cellular reprogramming, genome editing (CRISPR/Cas9) and vaccines. Polyplus-transfection® has developed a novel mRNA transfection reagent (jetMESSENGER®) which outperforms the efficiency of DNA transfection. This poster will present the results obtained with various cell lines, fragile and primary cells.
Need certificate of analysis ?
In order to download a product protocol or a certificate of analysis, please create an account on Polyplus®Portal .
Create an account to discover our
- Certificate of Analysis
- Plasmid Design Platform
- Product Protocols
- All exclusive gated content
Check our FAQs
These FAQs are organized by application to guide you to find the best answer possible.
You have access to all the documents related to the Polyplus Products such as MSDS, NHPS and Posters.
Search for publications in the Transfection Database with Polyplus transfection reagents or for transfection conditions. Over 6000 publications, 1000 cell lines and primary cells available.
This lexicon will help you to understand the different terms related to Polyplus products and services.