Abstract

Development process for biotherapeutic protein production usually begins with generating a high-performing stable cell line which can be used for manufacturing. As this step takes a lot of time, transient transfection offers a great alternative to quickly produce milligram to gram quantities of recombinant proteins and antibodies. A various number of culture media are available for performing transient protein production in both CHO and HEK cells, but the limiting factor often remains the transfection reagent. Therefore, Polyplus-transfection has developed a novel technologically advanced transfection solution named FectoPRO®. Here we show that FectoPRO® outperforms currently available PEI-based and lipid-based transfection reagents in all the transient expression systems tested, offering great transfection efficiency and amazing protein yields.