Protocol
- Description: Human embryonic kidney Fibroblast
Known as: HEK293, 293 - Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection Reagent | jetCRISPR, SpCas9 Nuclease |
---|---|
Plate format | 96-well plate |
Cell culture conditions | Pre-coated plate with 50µL fibronectine at 0.05 mg/mL and use a MEM culture medium + 1% NEAA (non-essential amino acids) + 10% FBS + 1% Glutamine + 2% Pen/Strep |
Transfection medium | Same as above |
Number of seeded cells/Confluency | Reverse: 20 000 cells/well or Forward: 12 000 cells/well |
Transfection volume | 125 µL |
Amount of biomolecule | 30 nM |
Amount of reagent | 0.3 µL |
Transfection analysis performed after | 48 h |