Protocol
- Description: Human cervix epidermoid carcinoma cells
Known as: CaSki - Delivered Biomolecule: DNA
Transfection Reagent | jetPEI |
---|---|
Plate format | 24-well plate |
Cell culture conditions | DMEM with 4.5 g/L glucose, 50 mM Hepes, 2 mM glutamine, antibiotics, 10% FBS |
Transfection medium | Same as above |
Number of seeded cells/Confluency | 60 – 70% |
Transfection volume | 0.5 ml |
Amount of biomolecule | 0.2 µg |
Amount of reagent | 1.6 µl |
Transfection analysis performed after | 24 h |