Protocol
- Description: Human lung carcinoma cells, type II pneumocytes
Known as: A-549 - Delivered Biomolecule: DNA
Transfection Reagent | jetOPTIMUS |
---|---|
Plate format | 24-well plate |
Cell culture conditions | Ham's F12 + 10% FBS + 2% Pen/Strep + 1% Glutamine |
Transfection medium | Complete Medium followed by a medium change 4h post-transfection with complete medium |
Number of seeded cells/Confluency | 60 000 cells |
Transfection volume | 500 µL |
Amount of biomolecule | 500 ng |
Amount of reagent | 0.5 µL |
Transfection analysis performed after | 24h |