FectoPRO is a transfection reagent specifically optimized for transient protein and antibody expression in CHO and HEK293 cells.
Bioreactor transfection is a method of deliberately introducing plasmids into large numbers of cultured cells for protein production The global COVID 19 health crisis caused by the severe acute respiratory syndrome coronavirus 2 (SARS CoV 2 demonstrated the imperativity to produce large quantities of vaccine components such as spike proteins, the predominant antigen of SARS CoV 2 S vaccines. To demonstrate feasibility, production of the SARS CoV 2 S HexaPro spike protein was conducted using the suspension adapted HEK 293 cell platform Expi 293 F ™transfected with spike protein encoding plasmid DNA. To achieve high yield, the transfection was performed using FectoPRO ® in a BioBLU ® 1 c Single Use Bioreactor controlled by the SciVario ® twin bioreactor control system
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