FectoCHO® expression system is optimized for culture and transient protein expression of all CHO cells.
The growing need in the biopharmaceutical market for various recombinant proteins and antibodies produced in a short time requires an efficient and flexible system. Transient expression is a commonly used process to face this demand but is unfortunately limited by transfection efficiency and inherent productivity, especially with CHO cells. To overcome this issue, we developed an advanced transient expression system consisting in the synergistic association of a novel CHO chemically defined medium and a powerful transfection reagent. First, we show that this innovative medium allows easy cultivation of various strains of CHO cells without the need of an extensive sequential adaptation. In a second time, through the protocol optimization of a technologically advanced transfection solution, we demonstrate a major increase in our recombinant proteins yields.
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