Viral vectors have become increasingly favored by researchers due to their efficiency in delivering genetic material into target cells and their ability for stable gene expression. Engineered to reduce immunogenicity, these vectors offer a safe means for delivering therapeutic genes, making them pivotal in gene therapy research and the development of vaccines.  

Achieving optimal use of viral vectors requires the establishment of a robust pipeline to produce them, a capability we proudly offer with jetOPTIMUS®. Indeed, jetOPTIMUS® streamlines your research process, enabling you to efficiently transfect DNA for an optimized small-scale virus production.  


Best-in-class DNA Transfection Reagent

  • A powerful transfection reagent that improves cellular uptake and endosomal escape of DNA resulting in higher transfection efficiency. 
  • Gentle on cells, preserving cell viability and morphology, which allows generation of biologically relevant data. 
  • Efficient in adherent cells and hard-to-transfect cells. 

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Plasmid Engineering Service 

As producing the perfect viral vector highly relies on good plasmid design and engineering, Polyplus® also provides a Plasmid Engineering Service to quickly optimize your DNA sequence. 

Thanks to our intuitive interface, you can create any kind of plasmid to fit your experimental needs. 

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Combining internal tests, article review and customer feedback, jetOPTIMUS® has proven its efficiency in adherent cells and hard-to-transfect cells such as primary cells, stem cells, cancer cell lines and neurons. 

Discover all the cell lines for which jetOPTIMUS® has provided good transfection results. 

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Article on Virus production with jetOPTIMUS® & Guide for Optimal DNA Transfection

jetOPTIMUS® enabled the reconstitution of r301Bs by transfecting DF-1-Cre cells with purified BAC DNA using our protocol. The transfected cells were then mixed and seeded with fresh primary CECs until plaques formed, then further propagated in CECs until virus stocks could be stored.  

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Understanding the underlying mechanisms and the critical parameters influencing DNA transfection is essential for achieving optimal, fast reproducible and reliable results. Polyplus® has put together a guide with 7 tips to help researchers master the art of DNA transfection. 

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Other Helpful Resources

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These FAQs are organized by application to guide you to find the best answer possible.

Product Documents

You have access to all the documents related to the transfection reagent.

Transfection Database

Search in the publication database with Polyplus transfection reagents or for transfection conditions

Our Support Specialists

For any technical inquiries, get in touch with our friendly team of PhD Scientific Support Specialists.

Discover Our Applications

Cell & Gene Therapy

Success of Gene and Cell Therapies is dependent on efficient production of viral vectors that require optimized transfection reagents and viral vector engineering

RNA/DNA Therapeutics

RNA/DNA in vivo delivery is the most powerful alternative to viral vectors for nucleic acid-based therapies. They offer substantial advantages in terms of reliability,safety and costs for nucleic-acid based therapies

Protein Production

Broad range of solutions is needed for manufacturing of functional  proteins or antibodies at the desired scale in bacteria, yeast and mammalian cell expression systems

Life Sciences

Optimised plasmid engineering and specific transfection reagents are key to enable protein expression in a wide range of adherent and suspension mammalian cell types