Protocol

• Description: Human embryonic kidney Fibroblast
  Known as: HEK293, 293
• Delivered Biomolecule: DNA

Transfection Reagent	jetOPTIMUS
Plate format	24 well-plate
Cell culture conditions	EMEM + 10% FBS + 2% Pen/Strep + 1% Glutamine + 1% AANE
Transfection medium	Complete Medium followed by a medium change 4h post-transfection with complete medium
Number of seeded cells/Confluency	50 000 cells
Transfection volume	500 µL
Amount of biomolecule	250 ng
Amount of reagent	0.38 µL
Transfection analysis performed after	24h