GP2-293 packaging cells (3.10^5) were seeded on 24-well plate and transfected with retroviral plasmid pLNCX2_eGFP using jetPRIME transfection reagent after 6 h from seeding. Supernatant containing retroviral particles was harvestedafter 48 h from the cells with highest eGFP production. For virus production (retrovirus).

Dendritic poly(L-lysines) (DGL) constitute promising nanomaterials applicable as a nonviral gene-delivery vector. In this study, we evaluate the transfection abilities of four DGL generations with special emphasis on the systematic description of the relationship of how generation (i.e., molecule size) affects the transfection efficacy. Using Hep2 cells, we demonstrated that the capability of unmodified DGL to deliver plasmid is of a magnitude lower than that of jetPEI. On the other hand, employing the Hep2 cell line stably transduced with eGFP, we observed that DGL G5 delivers the siRNA oligonucleotide with the same efficiency as Lipofectamine 2000. In further experiments, it was shown that DGL affords excellent ability to bind DNA, protect it against DNase I attack, and internalize it into cells.