HuH-7 cells (2×10^6) were seeded in a 10-cm diameter dish for 24 h before transfection. pNF-κB-tk-luc2 vector (8 μg) and 16 μl of jetPEI solution were diluted with 500 μl and 484 μl of 145 mM NaCl, respectively, then immediately mixed together and incubated for 30 minutes at room temperature. The 1000 μl jetPEI/DNA mixture was then added to the HuH-7 cells in the 10 cm diameter dish, and incubated at 37˚C for 24 hours.

Few studies have reported that the effect of sorafenib on advanced human hepatocellular carcinoma (HCC) is taking place via the inhibition of NF-