Protocol
- Description: Primary human hepatocytes
- Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection Reagent | jetCRISPR, SpCas9 Nuclease |
---|---|
Plate format | 96-well plate |
Cell culture conditions | Hepatocyte Maint. Media w/ Supplement (ref: MM250, Lonza) |
Transfection medium | Same as above |
Number of seeded cells/Confluency | Forward protocol: 40 000 cells/ well |
Transfection volume | 150 µL |
Amount of biomolecule | 30 nM |
Amount of reagent | 0.3 µL |
Transfection analysis performed after | 48 h |