Protocol
- Description: Human primary melanocyte cells
- Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
Transfection Reagent | jetCRISPR, SpCas9 Nuclease |
---|---|
Plate format | 96-well plate |
Cell culture conditions | Melanocytes Growth medium M2 |
Transfection medium | Reduced serum medium followed by a medium change 4h post-transfection with a complete medium (same as above) |
Number of seeded cells/Confluency | Forward: 7 500 cells/well |
Transfection volume | 125 µL |
Amount of biomolecule | 30 nM |
Amount of reagent | 0.3 µL |
Transfection analysis performed after | 48h |