Protocol
- Description: Human colon carcinoma epithelial cells
- Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
| Transfection Reagent | jetCRISPR, SpCas9 Nuclease |
|---|---|
| Plate format | 96-well plate |
| Cell culture conditions | DMEM 4,5 g/L glucose + 1% AANE + 1% Na Pyruvate + 20% FBS + 1% Glutamine + 2% Pen / Strep |
| Transfection medium | Same as Above |
| Number of seeded cells/Confluency | Reverse transfection: 20 000 cells/well |
| Transfection volume | 125 µL |
| Amount of biomolecule | 30 nM |
| Amount of reagent | 0.3 µL |
| Transfection analysis performed after | 48 h |