Protocol
- Description: Primary rat hippocampal neurons
- Delivered Biomolecule: mRNA
Transfection Reagent | jetMESSENGER |
---|---|
Plate format | 48-well plate |
Cell culture conditions | Neurobasal medium, 2% B27 50x, Glutamate 25µM |
Transfection medium | Same as cell culture medium |
Number of seeded cells/Confluency | Morphology : Cortex Neurons must be mature enough to develop dendrites - Density: 100 000 cells/well |
Transfection volume | 0.5 mL |
Amount of biomolecule | 150 ng |
Amount of reagent | 0,3 µl |
Transfection analysis performed after | 24 - 48h |