Protocol
- Description: Human acute monocytic leukaemia cells
Known as: THP1, THP 1 - Delivered Biomolecule: mRNA
| Transfection Reagent | jetMESSENGER |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | RPMI 1640, 10% FBS, 2mM glutamine, 1% P/S, 1% HEPES, 1% Sodium pyruvate, 0,05mM beta-mercaptoethanol |
| Transfection medium | Same as cell culture medium |
| Number of seeded cells/Confluency | Suspension cells - Density: 100 000 cells/well |
| Transfection volume | 0.5 mL |
| Amount of biomolecule | 500 ng |
| Amount of reagent | 1 µL |
| Transfection analysis performed after | 24h |