Protocol

  • Description: Human lung cells
  • Delivered Biomolecule: mRNA
Transfection Reagent jetMESSENGER
Plate format 24-well plate
Cell culture conditions

DMEM 1g/L glucose, 10% FBS, 2mM glutamine, 2% P/S, 1% NaPy, 1% AANE

Transfection medium Same as cell culture medium
Number of seeded cells/Confluency 60-80%
Transfection volume 0.5 mL
Amount of biomolecule 500 ng
Amount of reagent 1 µl
Transfection analysis performed after 24h