Protocol
- Description: Human anaplastic thyroid carcinoma cells
- Delivered Biomolecule: DNA
| Transfection Reagent | jetPEI |
|---|---|
| Plate format | 6-well plate |
| Cell culture conditions | RPMI 1640 medium, 80%; fetal bovine serum, 20% |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 250 000 cells per well |
| Transfection volume | 1 ml |
| Amount of biomolecule | 3 µg |
| Amount of reagent | 6 µl |
| Transfection analysis performed after | - |