Protocol
- Description: Primary rat hippocampal neurons
- Delivered Biomolecule: DNA
| Transfection Reagent | jetPEI |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Neurobasal medium + B-27 +1 mM sodium pyruvate + 5µg/ml insulin + 2 mM Glutamin + 100U/ml penicillin |
| Transfection medium | Neurobasal medium, without serum |
| Number of seeded cells/Confluency | - |
| Transfection volume | 1 ml |
| Amount of biomolecule | 1 µg |
| Amount of reagent | 3.6 µl |
| Transfection analysis performed after | - |