Protocol

• Description: Primary rat hippocampal neurons
• Delivered Biomolecule: siRNA

Transfection Reagent	INTERFERin
Plate format	24-well plate
Cell culture conditions	Neurobasal medium + B-27 +1 mM sodium pyruvate + 5µg/ml insulin + 2 mM Glutamin + 100U/ml penicillin
Transfection medium	Neurobasal medium, without serum
Number of seeded cells/Confluency	50 000 cells per well
Transfection volume	0.5 ml
Amount of biomolecule	50 nM
Amount of reagent	2 µl
Transfection analysis performed after	-