Protocol

  • Description: Primary mouse newborn astrocytes
  • Delivered Biomolecule: DNA
Transfection Reagent jetPRIME
Plate format 24-well plate
Cell culture conditions DMEM with 2 mM glutamine, antibiotics, 10% FBS
Transfection medium -
Number of seeded cells/Confluency 70 % confluent
Transfection volume 0.5 ml
Amount of biomolecule 0.5 µg
Amount of reagent 1.5 µl
Transfection analysis performed after -