Protocol
- Description: Murine lung SV40 transformed cells
Known as: MLE-15 - Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | RPMI with 2 mM glutamine, antibiotics, 2% FBS, 0,005 mg/ml insulin, 0,01 mg/ml transferrin, 30nM sodium selenite, 10 nM hydrocortisone, 10mM Hepes + béta oestradiol 0,01µM |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 30 - 50% |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 50 nM |
| Amount of reagent | 3 µl |
| Transfection analysis performed after | 48 h |