Protocol
- Description: Human immortalized microglial cells
Known as: - Delivered Biomolecule: DNA
| Transfection Reagent | jetPRIME |
|---|---|
| Plate format | 48-well plate |
| Cell culture conditions | DMEM containing 4.5 g/l glucose, 2 mM glutamine, antibiotics, 10% New born calf serum |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 90% |
| Transfection volume | 200 µl |
| Amount of biomolecule | 0.5 µg |
| Amount of reagent | 1 - 1.5 µl |
| Transfection analysis performed after | add 0.5 ml media after 4 h and analyse after 48 h |