Protocol

• Description: Human breast adenocarcinoma cells
  Known as: MCF-7, MCF 7
• Delivered Biomolecule: siRNA

Transfection Reagent	INTERFERin
Plate format	96-well plate
Cell culture conditions	MEM with Earle's salt, 2 mM glutamine, 1.5 g/l sodium bicarbonate, 0.1 mM NEAA, 1 mM sodium pyruvate, antibiotics, 10% FBS
Transfection medium	Same as above
Number of seeded cells/Confluency	7 500 cells per well
Transfection volume	125 µl
Amount of biomolecule	1 nM
Amount of reagent	0.5 µl
Transfection analysis performed after	48 h