Protocol
- Description: Human breast adenocarcinoma cells
Known as: MCF10A, MCF 10A - Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 6-well plate |
| Cell culture conditions | MEBM MEGM SingleQuot additives (w/o GA-1000), 100 ng/ml cholera toxin, 1% Pen/Strep |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 50 % confluent |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 5 nM |
| Amount of reagent | 4 µl |
| Transfection analysis performed after | 48 h |