Protocol
- Description: Primary human keratinocytes
Known as: human keratinocytes, keratinocytes - Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 6-well plate |
| Cell culture conditions | not specified |
| Transfection medium | not specified |
| Number of seeded cells/Confluency | 150 000 cells/well 12 hours before transfection |
| Transfection volume | 2.2 ml |
| Amount of biomolecule | 50 nM |
| Amount of reagent | 10 µl |
| Transfection analysis performed after | 72 h |