Protocol
- Description: Mouse embryonic fibroblastic cells
Known as: 10T1/2 - Delivered Biomolecule: DNA
| Transfection Reagent | jetPRIME |
|---|---|
| Plate format | 6-well plate |
| Cell culture conditions | DMEM with 4.5 g/L glucose, 50 mM Hepes, 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | DMEM 4.5 g/l glucose, 1% non-essential amino-acids, 2 mM glutamine, antibiotics, 10% FBS |
| Number of seeded cells/Confluency | 200 000 cells |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 2 µg |
| Amount of reagent | 5 µl |
| Transfection analysis performed after | not specified |