Protocol
- Description: Murine Lewis lung carcinoma cells
- Delivered Biomolecule: siRNA
Transfection Reagent | INTERFERin |
---|---|
Plate format | 12-well plate |
Cell culture conditions | DMEM containing 4.5 g/l glucose, 2 mM glutamine, antibiotics, 10% fetal calf serum |
Transfection medium | - |
Number of seeded cells/Confluency | 150 000 cells per well |
Transfection volume | 1 ml |
Amount of biomolecule | 1 nM |
Amount of reagent | 3 µl |
Transfection analysis performed after | 48 h |