Our fully integrated protein manufacturing services covers all needs from initial process development to manufacturing at research, HQ and GMP grade.
Meet our team during the Viral Vector & Vaccines meeting organized by ISBBiotech.
Addressing large-scale viral vector manufacturing using an optimized PEI-based transfection process
With the progress in developing new viral vector systems guided by safety, specificity and potency considerations, several gene and cell based therapies are now more than ever closer to being clinically approved and commercially available to treat genetic diseases. Viral vector delivery systems, of which mainly adeno-associated viruses (AAV) and lentiviruses are produced by transient transfection of mammalian producer HEK-293 cell lines. Virus vector production using the right transient transfection method is crucial to provide the flexibility and reproducibility that is needed to scale-up from initial process development to manufacturing of high-quality grade viral vectors.
Our solutions for virus production
PEIpro® is a DNA transfection reagent for production of viruses, recombinant proteins and antibodies either in adherent cells in presence of serum or in suspension HEK-293 cells grown in low serum or serum-free media.
PEIpro®-HQ is a highly qualified grade of PEIpro® reagent, especially developed for the production of clinical batches of recombinant proteins, antibodies or viral vectors. The PEIpro®-HQ grade is supplied with appropriate Quality Controls and documentation allowing its use as a qualified raw material in GMP processes for the manufacturing of clinical batches of therapeutic products.
FectoPRO® Transfection kit is specifically designed for enhanced Transient Gene Expression (TGE) in suspension CHO and HEK-293 cells in various serum-free media, using low DNA amount (< 1 µg/ml of cell culture).
The convention will take place in Norfolk, Virginia, United States:
Explore All our Products and Services
Explore our easy plasmid services starting at just $249. Our fully sequenced plasmids, ready for your research, include a miniprep. Reduce project cos...
pPLUS® AAV-Helper is a novel plasmid helper designed for AAV production. This plasmid has been optimized for triple transfection of HEK293 cells in s...
Polyplus® free of charge viral vector DOE service is the combination of our expertise in the field of nucleic acid delivery and in DoE methodology.
Polyplus offers a comprehensive range of plasmid manufacturing services from DNA plasmid design to plasmid DNA manufacturing and analytical testing.
Obtain accurate and reliable full length plasmid sequencing with our Next Generation Sequencing service.
Polyplus provides an integrated service for design and production of tailor-made DNA plasmid through our unique and Innovative assembly technology.
FectoVIR®-LV transfection reagent designed to improve Lentiviral vector (LV) titers in HEK-293 suspension cell systems.
LipidBrick® is a novel range of proprietary cationic lipids dedicated to the formulation of LNPs for the development of mRNA-based vaccines and thera...
in vivo-jetRNA®+ is a lipid-based transfection reagent composed of preformed liposomes specifically developed for in vivo mRNA delivery.
Available at research and GMP grade to intensify production of recombinant AAV rAAV viral vectors at any scale from benchtop to 2000L scale bioreactor...
in vivo-jetPEI® is a ready-to-use cationic polymer reagent recommended for in vivo transfection of DNA, siRNA, miRNA, shRNA and other oligonucleotide...
jetOPTIMUS® is an innovative cationic nanotechnology developed to improve DNA transfection efficiency in hard-to-transfect cells.
FectoCHO® expression system is optimized for culture and transient protein expression of all CHO cells.
PEIpro® is the leading PEI-based DNA transfection reagent that offers flexibility and scalability for viral vector manufacturing.
jetMESSENGER® is a designed for high mRNA transfection efficiency in primary cells, cancer cell lines, neurons, and stem cells.
High-throughput cDNA libraries screening is a valuable tool to study gene expression and regulation and protein interactions and functions.
FectoPRO is a transfection reagent specifically optimized for transient protein and antibody expression in CHO and HEK293 cells.
The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies in live cells.
INTERFERin® provides very high silencing efficiency already at 1 nM siRNA and can be used in a wide variety of adherent and suspension cells....