Protocol
- Description: Human acute monocytic leukaemia cells
Known as: THP1, THP 1 - Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | RPMI with 2 mM glutamine, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | not specified |
| Transfection volume | 0.2 ml |
| Amount of biomolecule | 20 nM |
| Amount of reagent | 3 µl |
| Transfection analysis performed after | 48 h |