Protocol

• Description: Human colon adenocarcinoma cells
• Delivered Biomolecule: siRNA

Transfection Reagent	INTERFERin
Plate format	96-well plate
Cell culture conditions	Leibovitz's L-15 medium with 10% Fetal Calf serum and antibiotics. 0% CO2
Transfection medium	Same as above
Number of seeded cells/Confluency	20 000 cells per well
Transfection volume	125 µl
Amount of biomolecule	10 nM
Amount of reagent	0.5 µl
Transfection analysis performed after	48 h