Protocol
- Description: Human ovary carcinoma cells
Known as: SKOV3, SKOV-3 - Delivered Biomolecule: DNA
| Transfection Reagent | jetPEI |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | Mc Coy's 5a modified medium, 1.5 mM L-glutamine, 2.2 g/L sodium bicarbonate, antibiotics, 10% FBS |
| Transfection medium | - |
| Number of seeded cells/Confluency | 60 – 70% |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 1 µg |
| Amount of reagent | 2 µl |
| Transfection analysis performed after | 24 h |