Protocol

  • Description: Human neuroblastoma cells
    Known as:
  • Delivered Biomolecule: DNA
Transfection Reagent jetPRIME
Plate format 24-well plate
Cell culture conditions

HAM F12 with 2 mM glutamine, antibiotics, 1% NEAA, 15% FBS

Transfection medium Same as above
Number of seeded cells/Confluency 150 000 cells per well
Transfection volume 0.5 mL
Amount of biomolecule 0.75 µg
Amount of reagent 1.5 µL
Transfection analysis performed after 24 h