Protocol
- Description: Human lung fibroblast cells
Known as: MRC5, MRC 5 - Delivered Biomolecule: DNA
| Transfection Reagent | jetPEI |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | MEM with Earle's salt, 2 mM glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, antibiotics, 10% FBS |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 60 – 70% |
| Transfection volume | 1 ml |
| Amount of biomolecule | 1 µg |
| Amount of reagent | 3.2 µl |
| Transfection analysis performed after | 24 h |