Protocol

• Description: Human breast adenocarcinoma cells
  Known as: MCF-7, MCF 7
• Delivered Biomolecule: mRNA

Transfection Reagent	jetMESSENGER
Plate format	24-well plate
Cell culture conditions	MEM with Earl's salt, 10% FBS, 2mM glutamine, 2% P/S, 1% AANE
Transfection medium	Same as cell culture medium
Number of seeded cells/Confluency	60-80%
Transfection volume	0.5 mL
Amount of biomolecule	500ng
Amount of reagent	1µL
Transfection analysis performed after	24h