Protocol
- Description: Primary human keratinocytes
Known as: NHEK - Delivered Biomolecule: RNP (Cas9 Protein + gRNA)
| Transfection Reagent | jetCRISPR, SpCas9 Nuclease |
|---|---|
| Plate format | 96-well plate |
| Cell culture conditions | Keratinocytes Growth medium 2 |
| Transfection medium | Reduced serum medium followed by a medium change 4h post-transfection with a complete medium (same as above) |
| Number of seeded cells/Confluency | Forward: 5 000 cells/well |
| Transfection volume | 125 µL |
| Amount of biomolecule | 30 nM |
| Amount of reagent | 0.3 µL |
| Transfection analysis performed after | 48h |