Protocol
- Description: Human hepatocarcinoma cells
Known as: Huh7, Huh 7 - Delivered Biomolecule: siRNA
| Transfection Reagent | INTERFERin |
|---|---|
| Plate format | 24-well plate |
| Cell culture conditions | DMEM containing 1 g/l glucose, 2 mM glutamine, antibiotics, 10% New born calf serum |
| Transfection medium | Same as above |
| Number of seeded cells/Confluency | 75 000 cells |
| Transfection volume | 0.5 ml |
| Amount of biomolecule | 10 nM |
| Amount of reagent | 1 µl |
| Transfection analysis performed after | 48 h |