Protocol
- Description: Human mesenchymal stem cells
- Delivered Biomolecule: mRNA
| Transfection Reagent | jetMESSENGER |
|---|---|
| Plate format | 96-well plate |
| Cell culture conditions | MSCBM, suppléments kit MSCGM SingleQuots |
| Transfection medium | Same as cell culture medium |
| Number of seeded cells/Confluency | 60-80% |
| Transfection volume | 0.125 mL |
| Amount of biomolecule | 150 ng |
| Amount of reagent | 0.5 µl |
| Transfection analysis performed after | 24h |