Protocol

• Description: Primary rat hippocampal neurons
• Delivered Biomolecule: mRNA

Transfection Reagent	jetMESSENGER
Plate format	48-well plate
Cell culture conditions	Neurobasal medium, 2% B27 50x, Glutamate 25µM
Transfection medium	Same as cell culture medium
Number of seeded cells/Confluency	Morphology : Cortex Neurons must be mature enough to develop dendrites - Density: 100 000 cells/well
Transfection volume	0.5 mL
Amount of biomolecule	150 ng
Amount of reagent	0,3 µl
Transfection analysis performed after	24 - 48h