FAQ: Product information - Polyplus-transfection

What type of compound is jetPRIME®?

jetPRIME® is a proprietary cationic polymer-based molecule, synthesized at Polyplus-transfection®.

jetPRIME® forms positively charged complexes with plasmid DNA, which penetrate the cell through endocytosis. Plasmid DNA is then released from the endosomes in the cytosol via the proton sponge mechanism. Subsequent nuclear import of plasmid DNA is mainly achieved once the nuclear envelope disappears during mitosis.

How can I store jetPRIME®?

jetPRIME® is a very stable molecule. jetPRIME® and its buffer should be stored at 5°C ± 3°C. to ensure long term stability.

Experiments were conducted in house to test the stability of jetPRIME®. After 48 hours at 50°C, jetPRIME® showed a similar transfection efficiency as when stored at 5°C ± 3°C.

jetPRIME® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 5°C ± 3°C.

How long can I use my jetPRIME® vial for?

Expiry date is indicated in the Certificate of Analysis available in “My Account”.

Where can I find product literature, such as the protocol of jetPRIME®?

You can find the protocol on the jetPRIME® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/jetprime/

All product literature is available on:

Product literature

Which type of compound is INTERFERin®?

INTERFERin® is a new proprietary formulation. It was developed for in vitro delivery of siRNA into mammalian cells grown adherent or in suspension. In many cell lines, INTERFERin® leads to more than 90% of silencing efficiency with as little as 1 nM of siRNA.

How can I store INTERFERin®?

INTERFERin® should be stored tightly capped at 5°C ± 3°C. INTERFERin® should not be frozen, as this strongly compromises its efficiency. We recommend discarding the frozen tube and using a new one.

How long can I use my INTERFERin® vial for?

Expiry date is indicated in the Certificate of Analysis available in “My Account”.

Where can I find product literature, such as the INTERFERin® protocol?

You can find the protocol on the INTERFERin® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/interferin/

All product literature is available on:

Product literature

Which type of compound is jetMESSENGER®?

jetMESSENGER® is a cationic lipid-based reagent developed by Polyplus-transfection®. This transfection reagent facilitates the entry of messenger RNA (mRNA) in mammalian cells, and allows high transfection efficiency to be reached, notably in hard to transfect cells.

How can I store jetMESSENGER®?

jetMESSENGER® and the provided mRNA buffer should be stored at 5°C ± 3°C to ensure long-term stability.

Experiments were conducted in house to test the stability of jetMESSENGER®. When stored 9 months at room temperature or at -20°C, jetMESSENGER® showed a similar transfection efficiency as if stored at 5°C ± 3°C.

How long can I use my jetMESSENGER® vial for?

Expiry date is indicated in the Certificate of Analysis available in “My Account”.

Where can I find product literature, such as the protocol of jetMESSENGER®?

You can find the protocol on the jetMESSENGER® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/jetmessenger/

All product literature is available on:

Product literature

Which type of compound is PEIpro®?

PEIpro® is composed of a linear polyethylenimine (PEI) whose molecular weight is proprietary information. Extensive optimization experiments have been performed to select a linear PEI for optimal DNA delivery with the lowest cytotoxicity in vitro.

PEIpro® is provided as an aqueous solution of highly pure and qualified linear PEI.

How can I store PEIpro®?

PEIpro® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 5°C ± 3°C to ensure long term stability.

How long can I use my PEIpro® vial for?

Expiry date is indicated in the Certificate of Analysis available in “My Account”.

How can I store FectoPRO® & FectoCHO™ CD Medium?

FectoPRO® can undergo an accidental freeze/thaw cycle without compromising the transfection efficiency. However, for long term storage we recommend storing the reagent at 5°C ± 3°C to ensure long term stability.

How long can I use my FectoPRO® & FectoCHO™ CD Medium vial for?

Expiry date is indicated in the Certificate of Analysis available in “My Account”.

Where can I find product literature such as the FectoPRO® & FectoCHO™ CD Medium protocol?

You can find the protocol on the FectoPRO® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/fectopro/

Which type of compound is in vivo-jetPEI®? What is the difference with jetPEI®?

jetPEI® (for in vitro use) and in vivo-jetPEI® are based on the same active molecule, however they have been specifically developed for each application. Thus they differ in formulation, concentration, purity and validation tests (in vivo-jetPEI® has been tested for absence of endotoxin and validated for in vivo gene delivery in mice).

How can I store in vivo-jetPEI®?

in vivo-jetPEI® is very stable. However, for long-term conservation, we recommend storing in vivo-jetPEI® at -20± 5°C upon reception.

How long can I use my in vivo-jetPEI® vial for?

Expiry date is indicated in the Certificate of Analysis available in “My Account”.

Where can I find product literature such as the in vivo-jetPEI® protocol?

You can find the protocol on the in vivo-jetPEI® webpage in “Protocol & Files”: https://www.polyplus-transfection.com/products/in-vivo-jetpei/

All product literature is available on:

Product literature

Which type of compound is PULSin?

PULSin contains a cationic amphiphile molecule capable of forming complexes with many proteins, including antibodies or peptides, resulting in overall positively charged complexes. These PULSin/proteins complexes bind to syndecans, a molecule present on virtually all cell surface membrane, allowing entry in the cell and ensuing escape of the protein from the endosome into the cytosol.